DCHAS-L Discussion List Archive
Date: Mon, 12 Jul 2010 14:36:59 +1000
Reply-To: DCHAS-L Discussion List <DCHAS-L**At_Symbol_Here**LIST.UVM.EDU>
Sender: DCHAS-L Discussion List <DCHAS-L**At_Symbol_Here**LIST.UVM.EDU>
From: Paul Dover <Paul.Dover**At_Symbol_Here**MONASH.EDU>
Subject: Re: Biological Indicators
In-Reply-To: <39D19992D8A3804B8C801976ACB45962111C2B**At_Symbol_Here**prk-exch-01.vcp.local>
I've used Sterikon=AE plus Bioindicator, so long as you have access to a
60=B0C water bath.
Simple, works well and, as Edward has pointed out below, uses Bacillus
stearothermophilus spores so it's 'gold standard'.
http://85.238.144.18/analytics/Micro_Manual/TEDISdata/prods/1_10274_0001_
0002.html gives you more info, but need to Google for your local
supplier.
Cheers, Paul
____________________________________________
Paul Dover
Resources Manager
Medicinal Chemistry and Drug Action
Monash Institute of Pharmaceutical Sciences
Monash University (Parkville Campus)
381 Royal Parade, Parkville
Victoria, 3052. Australia
Phone: +61 3 9903 9551
Fax: +61 3 9903 9143
Email: Paul.Dover**At_Symbol_Here**pharm.monash.edu.au
Web: http://www.pharm.monash.edu.au
-----Original Message-----
From: DCHAS-L Discussion List [mailto:DCHAS-L**At_Symbol_Here**list.uvm.edu] On Behalf Of
McGrath Edward J
Sent: Friday, 9 July 2010 5:17 AM
To: Paul Dover
Subject: Re: [DCHAS-L] Biological Indicators
The gold standard for this protocol is Bacillus stearothermophilus
endospores. This is a bacterium that only grows at temperatures above
56 degrees C (can't seem to do symbols!)
Autoclaving at 121 degrees C for 15 minutes is sufficient to kill the
spores. After autoclaving, you inoculate appropriate medium and
incubate in a hot water bath, with a control culture of un-autoclaved
spores (which should, of course, grow).
I'm afraid I have no idea of pricing. Another option is autoclave tape,
which reveals writing after autoclaving. You should use this with every
autoclave run (it usually says "autoclaved" or "sterile.") This is good
for showing which medium has been sterilized and which hasn't (assuming
the medium is not compromised.) The disadvantage to the tape is that
with age, the writing can appear without autoclaving.
Edward J. McGrath
Science Supervisor
Red Clay Consolidated School District
office: (302) 552-3768
-----Original Message-----
From: DCHAS-L Discussion List [mailto:DCHAS-L**At_Symbol_Here**list.uvm.edu] On Behalf Of
Beth Welmaker
Sent: Thursday, July 08, 2010 2:00 PM
To: DCHAS-L**At_Symbol_Here**LIST.UVM.EDU
Subject: [DCHAS-L] Biological Indicators
I am looking for a recommendation for simple Biological Indicators to
verify our autoclave sterilization effectiveness.
There are several vial styles to choose from. I don't want to waste
money on a poor product.
Thank you in advance!
Beth Welmaker, CCHO
Safety Officer
11350 SW Village Parkway - Third Floor
Port Saint Lucie, FL=A0 34987
772-345-4826
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