Date: Mon, 12 Jul 2010 14:36:59 +1000
Reply-To: DCHAS-L Discussion List <DCHAS-L**At_Symbol_Here**LIST.UVM.EDU>
Sender: DCHAS-L Discussion List <DCHAS-L**At_Symbol_Here**LIST.UVM.EDU>
From: Paul Dover <Paul.Dover**At_Symbol_Here**MONASH.EDU>
Subject: Re: Biological Indicators
In-Reply-To: <39D19992D8A3804B8C801976ACB45962111C2B**At_Symbol_Here**prk-exch-01.vcp.local>

I've used Sterikon=AE plus Bioindicator, so long as you have access to a 
60=B0C water bath.
Simple, works well and, as Edward has pointed out below, uses Bacillus 
stearothermophilus spores so it's 'gold standard'.
0002.html gives you more info, but need to Google for your local 

Cheers, Paul


Paul Dover
Resources Manager
Medicinal Chemistry and Drug Action
Monash Institute of Pharmaceutical Sciences
Monash University (Parkville Campus)
381 Royal Parade, Parkville
Victoria, 3052. Australia

Phone: +61 3 9903 9551
Fax:   +61 3 9903 9143

Email: Paul.Dover**At_Symbol_Here**

-----Original Message-----
From: DCHAS-L Discussion List [mailto:DCHAS-L**At_Symbol_Here**] On Behalf Of 
McGrath Edward J
Sent: Friday, 9 July 2010 5:17 AM
To: Paul Dover
Subject: Re: [DCHAS-L] Biological Indicators

The gold standard for this protocol is Bacillus stearothermophilus 
endospores.  This is a bacterium that only grows at temperatures above 
56 degrees C (can't seem to do symbols!)

Autoclaving at 121 degrees C for 15 minutes is sufficient to kill the 
spores.  After autoclaving, you inoculate appropriate medium and 
incubate in a hot water bath, with a control culture of un-autoclaved 
spores (which should, of course, grow). 

I'm afraid I have no idea of pricing.  Another option is autoclave tape, 
which reveals writing after autoclaving.  You should use this with every 
autoclave run (it usually says "autoclaved" or "sterile.")  This is good 
for showing which medium has been sterilized and which hasn't (assuming 
the medium is not compromised.)  The disadvantage to the tape is that 
with age, the writing can appear without autoclaving.

Edward J. McGrath
Science Supervisor
Red Clay Consolidated School District
office:  (302) 552-3768
-----Original Message-----
From: DCHAS-L Discussion List [mailto:DCHAS-L**At_Symbol_Here**] On Behalf Of 
Beth Welmaker
Sent: Thursday, July 08, 2010 2:00 PM
To: DCHAS-L**At_Symbol_Here**LIST.UVM.EDU
Subject: [DCHAS-L] Biological Indicators

I am looking for a recommendation for simple Biological Indicators to 
verify our autoclave sterilization effectiveness.
There are several vial styles to choose from.  I don't want to waste 
money on a poor product. 

Thank you in advance!

Beth Welmaker, CCHO
Safety Officer

11350 SW Village Parkway - Third Floor
Port Saint Lucie, FL=A0 34987

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