Date: Wed, 27 Jul 2011 13:02:06 -0700
Reply-To: DCHAS-L Discussion List <DCHAS-L**At_Symbol_Here**LIST.UVM.EDU>
Sender: DCHAS-L Discussion List <DCHAS-L**At_Symbol_Here**LIST.UVM.EDU>
From: Eric Clark <erclark**At_Symbol_Here**PH.LACOUNTY.GOV>
Subject: Re: PCR benchtop ventilation?
At the LA County Public Health Lab there are a few PCR arrays,
including Light Cycler, Smart Cycler, ABI 7500, and Luminex. All
were installed by professional field engineers, and other than internal
fans to transfer heat, there is no need for ventilation.
Eric Clark, MS, CCHO, CHMM
Safety & Compliance Officer
Los Angeles County Public Health Laboratory
>>> On 7/27/2011 at 10:38 AM, in message <B9C496515E843746B
60EA72DA38D8CA8356169A430**At_Symbol_Here**MBXA.exchange.cornell.edu>, Ralph B Stuart
I was touring some labs
yesterday and noticed a variety of small benchtop machines that were
designed to do Polymerase Chain Reaction chemistry. Some of the references
I reviewed on this technique listed a variety of hazardous chemicals
associated with this process (phenol, chloroform, etc.), but they are
somewhat dated and I wonder if these machines use these chemicals.
The immediate question is what level of ventilation these devices
need. From what I can see in the instrument manuals I downloaded, the
ventilation needs seem to be limited to temperature control considerations,
but I thought I would check with the DCHAS list to see if anyone has
experience with chemical exhausts from these systems.
any help with this question.
Laboratory Ventilation Specialist