Date: Wed, 27 Jul 2011 14:27:50 -0400
Reply-To: DCHAS-L Discussion List <DCHAS-L**At_Symbol_Here**LIST.UVM.EDU>
Sender: DCHAS-L Discussion List <DCHAS-L**At_Symbol_Here**LIST.UVM.EDU>
From: Paul Harrison <pharriso**At_Symbol_Here**UNIVMAIL.CIS.MCMASTER.CA>
Subject: Re: PCR benchtop ventilation?
In-Reply-To: <B9C496515E843746B60EA72DA38D8CA8356169A430**At_Symbol_Here**>


The actual PCR process is pretty innocuous, and does not involve any volatile or toxic chemicals. The phenol and chloroform might be used subsequently on the samples, but not in the machine. I cannot really see why the machine itself would need to be in a hood. Hope this helps. On Wed, 27 Jul 2011 13:38:41 -0400 Ralph B Stuart wrote: > I was touring some labs yesterday and noticed a variety of small benchtop machines that were designed to do Polymerase Chain Reaction chemistry. Some of the references I reviewed on this technique listed a variety of hazardous chemicals associated with this process (phenol, chloroform, etc.), but they are somewhat dated and I wonder if these machines use these chemicals. > > The immediate question is what level of ventilation these devices need. From what I can see in the instrument manuals I downloaded, the ventilation needs seem to be limited to temperature control considerations, but I thought I would check with the DCHAS list to see if anyone has experience with chemical exhausts from these systems. > > Thanks for any help with this question. > > - Ralph > > > Ralph Stuart CIH > Laboratory Ventilation Specialist > rstuart**At_Symbol_Here** Paul Harrison Associate Professor of Chemistry Department of Chemistry and Chemical Biology McMaster University 1280 Main St. W., Hamilton, ON, L8S 4M1, Canada Phone: (905)525-9140 ext. 27290; FAX: (905)522-2509

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