We asked the teacher at a RCRA training about infectious cultures in agar. She said that once they have been autoclaved they are no longer infectious. Then you just have agar, which can be thrown away. Looks like some of your media have more than just organisms and agar, though. Dean Turner Shorter College -----Original Message----- From: DCHAS-L Discussion List [mailto:DCHAS-L**At_Symbol_Here**LIST.UVM.EDU] On Behalf Of Burrill, Jason Sent: Wednesday, October 10, 2007 10:21 AM To: DCHAS-L**At_Symbol_Here**LIST.UVM.EDU Subject: [DCHAS-L] Cell Culture Media Disposal I was wondering if anyone had any experience in trying to determine how to dispose of liquid cell culture media, whether it should be rendered non-infectious by autoclaving, disposed of as biological waste or taken away by a hazardous waste hauler as non-hazardous waste. I have listed the constituents below for reference and any help will be appreciated. Thanks in advance, Jason Constituent Percentage Fetal Bovine Serum 7.5-10% Fetal Calf Serum 7.5-10% Penicillin/Streptomycin Solution <0.1% L-Glutamine <0.1% MEM Non-Essential Amino Acids (100X) <0.1% Nucleosides <0.1% 2-Mercaptoethanol <0.1% Trypsin/EDTA solution 0.003-0.005% DMSO 0-0.000005% Water 70-80% Jason Burrill Manager, Histology Charles River Laboratories 251 Ballardvale Street Wilmington, MA 01887 Ph: 978-658-6000 ext 1652 Fax: 978-988-8793 jason.burrill**At_Symbol_Here**crl.com
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