Date: Wed, 10 Oct 2007 16:35:50 -0700
Reply-To: "Saldivar, Jay" <SaldivarJ**At_Symbol_Here**SHARPSEC.COM>
Sender: DCHAS-L Discussion List <DCHAS-L**At_Symbol_Here**LIST.UVM.EDU>
From: "Saldivar, Jay" <SaldivarJ**At_Symbol_Here**SHARPSEC.COM>
Subject: Re: Cell Culture Media Disposal
Comments: To: "Burrill, Jason"
In-Reply-To: A<1AD4E907E9B6F648AEF1B3A20A9B0E1E67322E**At_Symbol_Here**>

I don't have any answers addressing this question. However I have a question I believe is of a similar nature. For the first time our company's chem lab is looking at using Streptavidin Cy5, Oligo-DNA, Immunopure Streptavidin, and a lot of other 'bio-type' materials. Our evaluation process traditionally handles chemical hazmats and we have no experience with bios. Our scientists/bio-experts assure us that the hazards are 'minimal', but we're used to hearing this from them whether they're requesting baking soda or diborane. What precautions or concerns should I be focusing on in evaluating these materials from an EHS perspective? Thanks in advance, I. Jay Saldivar Sr Safety Administrator Sharp Microelectronics of the Americas NW Pacific Rim Blvd Camas, WA 98607 360/834-8734 (o) 360/772-4502 (c) saldivarj**At_Symbol_Here** -----Original Message----- From: DCHAS-L Discussion List [mailto:DCHAS-L**At_Symbol_Here**LIST.UVM.EDU] On Behalf Of Burrill, Jason Sent: Wednesday, October 10, 2007 10:21 AM To: DCHAS-L**At_Symbol_Here**LIST.UVM.EDU Subject: [DCHAS-L] Cell Culture Media Disposal I was wondering if anyone had any experience in trying to determine how to dispose of liquid cell culture media, whether it should be rendered non-infectious by autoclaving, disposed of as biological waste or taken away by a hazardous waste hauler as non-hazardous waste. I have listed the constituents below for reference and any help will be appreciated. Thanks in advance, Jason Constituent Percentage Fetal Bovine Serum 7.5-10% Fetal Calf Serum 7.5-10% Penicillin/Streptomycin Solution <0.1% L-Glutamine <0.1% MEM Non-Essential Amino Acids (100X) <0.1% Nucleosides <0.1% 2-Mercaptoethanol <0.1% Trypsin/EDTA solution 0.003-0.005% DMSO 0-0.000005% Water 70-80% Jason Burrill Manager, Histology Charles River Laboratories 251 Ballardvale Street Wilmington, MA 01887 Ph: 978-658-6000 ext 1652 Fax: 978-988-8793 jason.burrill**At_Symbol_Here**

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