DCHAS-L Discussion List Archive
Date: Wed, 10 Oct 2007 13:20:40 -0400
Reply-To: "Burrill, Jason" <Jason.Burrill**At_Symbol_Here**CRL.COM>
Sender: DCHAS-L Discussion List <DCHAS-L**At_Symbol_Here**LIST.UVM.EDU>
From: "Burrill, Jason" <Jason.Burrill**At_Symbol_Here**CRL.COM>
Subject: Cell Culture Media Disposal
I was wondering if anyone had any experience in trying to determine how
to dispose of liquid cell culture media, whether it should be rendered
non-infectious by autoclaving, disposed of as biological waste or taken
away by a hazardous waste hauler as non-hazardous waste. I have listed
the constituents below for reference and any help will be appreciated.
Thanks in advance,
Jason
Constituent
Percentage
Fetal Bovine Serum
7.5-10%
Fetal Calf Serum
7.5-10%
Penicillin/Streptomycin Solution
<0.1%
L-Glutamine
<0.1%
MEM Non-Essential Amino Acids (100X) <0.1%
Nucleosides
<0.1%
2-Mercaptoethanol
<0.1%
Trypsin/EDTA solution
0.003-0.005%
DMSO
0-0.000005%
Water
70-80%
Jason Burrill
Manager, Histology
Charles River Laboratories
251 Ballardvale Street
Wilmington, MA 01887
Ph: 978-658-6000 ext 1652
Fax: 978-988-8793
jason.burrill**At_Symbol_Here**crl.com
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