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Subject: Re: Safety Cards
Date: Wed, 10 Oct 2007 09:44:47 -0700
Author: Sheila Kennedy 		Next by Date:
Subject: Re: Cell Culture Media Disposal
Date: Wed, 10 Oct 2007 16:35:50 -0700
Author: "Saldivar, Jay"


Date: Wed, 10 Oct 2007 13:20:40 -0400
Reply-To: "Burrill, Jason" <Jason.Burrill**At_Symbol_Here**CRL.COM>
Sender: DCHAS-L Discussion List <DCHAS-L**At_Symbol_Here**LIST.UVM.EDU>
From: "Burrill, Jason" <Jason.Burrill**At_Symbol_Here**CRL.COM>
Subject: Cell Culture Media Disposal
I was wondering if anyone had any experience in trying to determine how
to dispose of liquid cell culture media, whether it should be rendered
non-infectious by autoclaving, disposed of as biological waste or taken
away by a hazardous waste hauler as non-hazardous waste. I have listed
the constituents below for reference and any help will be appreciated.

 

Thanks in advance,

Jason

 

Constituent
Percentage

Fetal Bovine Serum
7.5-10%

Fetal Calf Serum
7.5-10%

Penicillin/Streptomycin Solution
<0.1%

L-Glutamine
<0.1%

MEM Non-Essential Amino Acids (100X)                   <0.1%

Nucleosides
<0.1%

2-Mercaptoethanol
<0.1%

Trypsin/EDTA solution
0.003-0.005%

DMSO
0-0.000005%

Water
70-80%

 

 

Jason Burrill

Manager, Histology

Charles River Laboratories

251 Ballardvale Street

Wilmington, MA 01887

Ph: 978-658-6000 ext 1652

Fax: 978-988-8793

jason.burrill**At_Symbol_Here**crl.com

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