Date: Thu, 11 Oct 2007 10:04:42 -0400
Reply-To: dturner**At_Symbol_Here**SHORTER.EDU
Sender: DCHAS-L Discussion List <DCHAS-L**At_Symbol_Here**LIST.UVM.EDU>
From: dturner**At_Symbol_Here**SHORTER.EDU
Subject: Re: Cell Culture Media Disposal
In-Reply-To: A<0CF12FA2E59EA946ABBD5B23DADC178A03A34DA8**At_Symbol_Here**WADESEVS01.sma.sharpamericas.com>
We asked the teacher at a RCRA training about infectious cultures in
agar. She said that once they have been autoclaved they are no longer
infectious. Then you just have agar, which can be thrown away. Looks
like some of your media have more than just organisms and agar, though.
Dean Turner
Shorter College
-----Original Message-----
From: DCHAS-L Discussion List [mailto:DCHAS-L**At_Symbol_Here**LIST.UVM.EDU] On Behalf Of
Burrill, Jason
Sent: Wednesday, October 10, 2007 10:21 AM
To: DCHAS-L**At_Symbol_Here**LIST.UVM.EDU
Subject: [DCHAS-L] Cell Culture Media Disposal
I was wondering if anyone had any experience in trying to determine how
to dispose of liquid cell culture media, whether it should be rendered
non-infectious by autoclaving, disposed of as biological waste or taken
away by a hazardous waste hauler as non-hazardous waste. I have listed
the constituents below for reference and any help will be appreciated.
Thanks in advance,
Jason
Constituent
Percentage
Fetal Bovine Serum
7.5-10%
Fetal Calf Serum
7.5-10%
Penicillin/Streptomycin Solution
<0.1%
L-Glutamine
<0.1%
MEM Non-Essential Amino Acids (100X) <0.1%
Nucleosides
<0.1%
2-Mercaptoethanol
<0.1%
Trypsin/EDTA solution
0.003-0.005%
DMSO
0-0.000005%
Water
70-80%
Jason Burrill
Manager, Histology
Charles River Laboratories
251 Ballardvale Street
Wilmington, MA 01887
Ph: 978-658-6000 ext 1652
Fax: 978-988-8793
jason.burrill**At_Symbol_Here**crl.com
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