Tilak,

We would treat the culture dishes as a hazardous waste because of the wortmannin which is listed as highly toxic. If I was disposing of a large ongoing stream of similar material I would look at it more closely (TCLP it,  incinerate as bio-waste possibly)



On Oct 11, 2011, at 12:05 PM, Tilak Chandra wrote:

Dear CHAS Colleagues:

What are the best practices for disposing the toxic chemicals/toxins from the

culture? Autoclaving is standard practice for deactivating the cells; however

some chemicals cannot be deactivated using autoclaving. Specifically, the

cultures have been treated with wortmanin and p-nitrophenyl-B-D-gluco

pyranoside. I cannot find any information on disposal for these. Is there is any

certain method for the inactivation/disposal of such system?

Thank you very much.

Tilak

Tilak Chandra, Ph.D.

Chemical Safety Specialist

Facilities Planning & Management

Environment, Health and Safety

30 East Campus Mall

Madison, WI 53715

Ph. 608-890-0255

FAX 608-262-6767

tchandra**At_Symbol_Here**fpm.wisc.edu

Chuck Cooper
Senior Advisor for Research Safety
CHO, RSO, BSO
Environmental Health and Safety
Portland State University
503-725-4312
cooperc**At_Symbol_Here**pdx.edu