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Subject: Re: [DCHAS-L] Dilute Ethidium Bromide Disposal in California

Date: Aug 10, 2018 00:04 UTC

Author: Debbie M. Decker <dmdecker**At_Symbol_Here**UCDAVIS.EDU>

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Subject: [DCHAS-L] Chemical Safety headlines (14 articles)

Date: Aug 10, 2018 11:12 UTC

Author: DCHAS Membership Chair <membership**At_Symbol_Here**DCHAS.ORG>

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From: John Darius Soltes <jdarsoltes**At_Symbol_Here**HOTMAIL.COM>

Subject: Re: [DCHAS-L] Dilute Ethidium Bromide Disposal in California

Date: Aug 10, 2018 06:14 UTC

Reply-To: ACS Division of Chemical Health and Safety

In-Reply-To:  

Demystify: 

So, if I am not mistaken 0.5 ug/ml is 0.197 mols per liter EtBr


this is an abstract from 

Ethidium bromide: destruction and decontamination of solutions. (Armour Method ( Lunn, G.and E. Sansone)


Ethidium bromide in water, TBE buffer, Mops buffer, and cesium chloride solution may be completely degraded by reaction with sodium nitrite and hypophosphorous acid. Only non-mutagenic reaction mixtures were produced. Destruction was greater than 99.8% in all cases; the limit of detection was 0.5 micrograms ethidium bromide per milliliter of solution. Ethidium bromide also may be removed completely from the above solutions by using Amberlite XAD-16 resin. The limit of detection was 0.05 micrograms ethidium bromide per milliliter of solution (0.27 micrograms/ml when cesium chloride solution was used).



I haven't tested this next method but maybe follow this reaction at slight molar excess of the neutralizing reagents.


Lunn & Sansone Method: For each 100 ml of aqueous EtBr solution:

• Add 5% hypophosphorous acid.
• Add 12 ml of 0.5 M sodium nitrite.
• Stir briefly and let stand for 20 hours.
• Adjust pH to 7-9 using sodium hydroxide.
• Pour down drain with copious amounts of water.






-Hope this helps




John Darius Soltes CHMM











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