Varricchio,
The bench work might put double digit ppm levels into the air. The toxicology is rather low at about 150 to 300 mg/Kg regardless of route.
LD50 Mouse ip 200 mg/kg
[European Chemicals Bureau; IUCLID Dataset, 2- Mercaptoethanol (60-24-2) (2000 CD-ROM edition). Available from, as of June 6, 2005: http://esis.jrc.ec.europa.eu/] **PEER REVIEWED**
LD50 Mouse iv 480 mg/kg
[European Chemicals Bureau; IUCLID Dataset, 2- Mercaptoethanol (60-24-2) (2000 CD-ROM edition). Available from, as of June 6, 2005: http://esis.jrc.ec.europa.eu/] **PEER REVIEWED**
LD50 Mouse oral 190 mg/kg bw
[European Chemicals Bureau; IUCLID Dataset, 2- Mercaptoethanol (60-24-2) (2000 CD-ROM edition). Available from, as of June 6, 2005: http://esis.jrc.ec.europa.eu/] **PEER REVIEWED**
LD50 Rat oral 244 mg/kg
[Lewis, R.J. Sax's Dangerous Properties of Industrial Materials. 9th ed. Volumes 1-3. New York, NY: Van Nostrand Reinhold, 1996., p. 2113] **PEER REVIEWED**
LD50 Rabbit dermal 150 mg/kg
[Lewis, R.J. Sax's Dangerous Properties of Industrial Materials. 9th ed. Volumes 1-3. New York, NY: Van Nostrand Reinhold, 1996., p. 2113] **PEER REVIEWED**
LD50 Guinea pig dermal 300 mg/kg
[Lewis, R.J. Sax's Dangerous Properties of Industrial Materials. 9th ed. Volumes 1-3. New York, NY: Van Nostrand Reinhold, 1996., p. 2113] **PEER REVIEWED**
I am just trying to find out if anyone else does bench work with BME and allows the odor to permeate the lab until the procedure is finished?
Thanks to all,
James
-----Original Message-----
From: ACS Division of Chemical Health and Safety [mailto:DCHAS-L**At_Symbol_Here**PRINCETON.EDU] On Behalf Of Varricchio
Sent: Tuesday, March 26, 2019 3:20 PM
To: DCHAS-L**At_Symbol_Here**PRINCETON.EDU
Subject: Re: [DCHAS-L] â-mercaptoethanol (âME)
BME in the electrophoresis buffer??
I thought that given the odiferousness no one could tolerate a toxic amount.
Sent from my iPad , Fred and Claudette
> On Mar 26, 2019, at 2:21 PM, Wilhelm, Monique <mwilhelm**At_Symbol_Here**umflint.edu> wrote:
>
> We use double containment in ventilated storage and only use it in the hood, leaving all pipet tips and such in the hood to avoid volatility issues. It can cause a false hazard.
>
> Monique Wilhelm
> Laboratory Manager
> Department of Chemistry & Biochemistry University of Michigan - Flint
>
>
> -----Original Message-----
> From: ACS Division of Chemical Health and Safety
> [mailto:DCHAS-L**At_Symbol_Here**PRINCETON.EDU] On Behalf Of Stuart, Ralph
> Sent: Tuesday, March 26, 2019 2:10 PM
> To: DCHAS-L**At_Symbol_Here**PRINCETON.EDU
> Subject: Re: [DCHAS-L] â-mercaptoethanol (âME)
>
>> Not pleasant, but was it a hazard?
>
> The odor of BME beyond "not pleasant" - it's disruptive. I had an experience at a previous institution where fugitive BME odors led to 3 building-wide fire alarms in a year as people thought the smells were related to natural gas leaks. People who don't use BME in the hood to control fugitive odors risk having their (and others') work interrupted for significant amounts of time while the fire department investigates these calls.
>
> This is a case when looking at the properties of a chemical in isolation leads to missing key aspects of the situation.
>
> - Ralph
>
> Ralph Stuart, CIH, CCHO
> Environmental Safety Manager
> Keene State College
> 603 358-2859
>
> ralph.stuart**At_Symbol_Here**keene.edu
>
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