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Subject:
Re: [DCHAS-L] Old Bleach
Date:
Oct 25, 2021 21:51 UTC
Author:
JOHN L STRAUGHN <0000120dde6ec15c-dmarc-request**At_Symbol_Here**LISTS.PRINCETON.EDU>
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Subject:
Re: [DCHAS-L] Old Bleach
Date:
Oct 25, 2021 22:12 UTC
Author:
MN Cooper <00000313ede34dce-dmarc-request**At_Symbol_Here**LISTS.PRINCETON.EDU>
From:
Jason Fritz <lokster.jf**At_Symbol_Here**GMAIL.COM>
Subject:
Re: [DCHAS-L] Old Bleach
Date:
Oct 25, 2021 22:05 UTC
Reply-To:
ACS Division of Chemical Health and Safety
In-Reply-To:
[DCHAS-L] Old Bleach
Hello Patricia,
For long-term experimental studies, we had to prepare and track HOCl concentrations in solution over time, which was quite easy to do by measuring the absorbance at 292nm using a standard UV/Vis instrument. This may not work due to interference in complex buffers, but in simple aqueous systems, it worked quickly and was robust.
It should be fairly easy to prepare a dilution curve from a known stock container, estimate the amount of HOCl present in the old bleach containers, and then dilute as necessary to achieve a desired concentration for disinfection.
Perkin Elmer has a short lab resource sheet describing this method: https://resources.perkinelmer.com/lab-solutions/resources/docs/APP_Quantification-of-Sodium-Hypochlorite.pdf
Best of luck,
Jason
Hello,
I was wondering about using old stocks of bleach for disinfecting in bio labs. The bleach is about 2 years old and has never been opened. I am not sure if it is industrial or commercially available bleach. Any advice would be appreciated.
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Patricia Leach
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Environmental Health and Safety Coordinator
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Emergency Management and Safety
Texas A&M University - Commerce
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P: 903.468.3278
C: 316.644.8255
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Mail: P.O. Box 3011, Commerce, TX 75429
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