DCHAS-L Archives, Jun 21, 2022 - Re: [DCHAS-L] [External] Re: [DCHAS-L] [External] Re: [DCHAS-L] Qiagen kits incident report

From: Shannon Nephew <millersc**At_Symbol_Here**PLATTSBURGH.EDU>

Subject: Re: [DCHAS-L] [External] Re: [DCHAS-L] [External] Re: [DCHAS-L] Qiagen kits incident report

Date: Jun 21, 2022 23:33 UTC

Reply-To: ACS Division of Chemical Health and Safety

In-Reply-To: Re: [DCHAS-L] [External] Re: [DCHAS-L] Qiagen kits incident report

Hi Ralph,

There is a comment in the enclosed booklet with the kit that has bulleted warning labels, but this is the only one I could find online at the moment...I show the actual booklet we used, when I talk about this in training. I ask if anyone knows what is generated if you mix ethanol (which is in the sample prep waste) with bleach? We then discuss chloroform...

"Do not add bleach or acidic solutions directly to the sample preparation waste. CAUTION: The sample preparation waste contains guanidinium hydrochloride from Buffer BG3, which can form highly reactive compounds when combined with bleach. For more information please consult the appropriate material safety data sheets (MSDS)."

SHANNON C. NEPHEW,

MS, CSM, CCHO

Chemical Hygiene Officer, Hudson Hall Science Complex Building Manager

Science Programs and Facilities Support Professional

Hudson Hall 317

101 Broad Street
Plattsburgh, NY 12901

(o) 518-593-9612

plattsburgh.edu

On Tue, Jun 21, 2022 at 5:38 PM Ralph Stuart <ralph**At_Symbol_Here**rstuartcih.org> wrote:

> we flicked the waste tubes into a disposal beaker containing bleach and very shortly, my postdoc and myself felt very lightheaded and dizzy. Once one of us mentioned it and realized we both felt this way, we immediately left the lab and mentally retraced our steps to determine what was going on.

Not a chlorine exposure then?

- Ralph

Ralph Stuart, CIH, CCHO
ralph**At_Symbol_Here**rstuartcih.org

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