Date: Wed, 22 Aug 2007 18:38:01 -0400
Reply-To: Ralph Stuart <rstuart**At_Symbol_Here**UVM.EDU>
Sender: DCHAS-L Discussion List <DCHAS-L**At_Symbol_Here**LIST.UVM.EDU>
From: Ralph Stuart <rstuart**At_Symbol_Here**UVM.EDU>
Subject: LN2 for tissue storage

A lab tech asked us this today and I thought that DCHAS-L might know  
more than we could find on google:

We recently purchased a LN2 cryo tank for storing tissue samples.   
The study requirement is that the tissue samples be stored in the  
liquid phase of LN2.  So far all the folks that I have spoken to  
around here store in the vapor phase - not liquid.  I have been  
searching online trying to find information on user safety protocols,  
PPE, and any other helpful pointers for adding and retrieving samples  
from liquid LN2, but not finding as much details as we would like.   
Do you know anyone who stores samples in the liquid phase of LN2?   
Or, any other suggestions?

Thanks for any information.

- Ralph

Ralph Stuart, CIH
Environmental Safety Manager
University of Vermont
Environmental Safety Facility
667 Spear St. Burlington, VT  05405

fax: (802)656-5407

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