Date: Thu, 23 Aug 2007 10:43:58 -0400
Reply-To: "Jackson, William" <William.Jackson**At_Symbol_Here**PGWORKS.COM>
Sender: DCHAS-L Discussion List <DCHAS-L**At_Symbol_Here**LIST.UVM.EDU>
From: "Jackson, William" <William.Jackson**At_Symbol_Here**PGWORKS.COM>
Subject: Re: LN2 for tissue storage
Comments: To: "Wawzyniecki Jr, Stefan"
In-Reply-To: A<72F919DD7D07B440A4B45FA2B57EAC45197C19**At_Symbol_Here**>

Members: there are also cryogenic aprons available. -----Original Message----- From: DCHAS-L Discussion List [mailto:DCHAS-L**At_Symbol_Here**LIST.UVM.EDU] On Behalf Of Wawzyniecki Jr, Stefan Sent: Thursday, August 23, 2007 9:52 AM To: DCHAS-L**At_Symbol_Here**LIST.UVM.EDU Subject: Re: [DCHAS-L] LN2 for tissue storage Here are 2 comments from our researchers... ________________________________ To: 'DCHAS-L**At_Symbol_Here**LIST.UVM.EDU' Subject: LN2 for tissue storage "I have been storing samples in liquid nitrogen for many years. The manufacturers of cryovials say that they can explode if stored in the liquid phase (i.e., when trapped liquid expands on warming), but in 25 years of doing just this, I have never seen it happen. I usually leave the vial lids slightly loose to allow escape of gas, but some people I know punch tiny holes in the lids as a precaution. I generally wear nitrile gloves (usually double gloves) to remove cryovials and canes from the storage dewar, and transfer canes to low styrofoam containers filled with liquid nitrogen to manipulate or sort samples. Hemostates work well for grabbing vials and lids to unscrew. In all these years I have occasionally had minor frostbite on my fingertips, but nothing worse." ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ ~~~~~~~~~~~~~~~~~~~~~~~~~ "We routinely store cells in the liquid phase of LN2. PPE includes a face shield and the cryo gloves sold by Fisher and others (easily found in the LN2 sections of the Fisher catalogue), as well as a lab coat. Vials are stored in racks, which we let drain when we pull them out of the liquid phase of LN2. Any remaining liquid will evaporate rapidly when at air temperature." ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ To: DCHAS-L**At_Symbol_Here**LIST.UVM.EDU Subject: [DCHAS-L] LN2 for tissue storage A lab tech asked us this today and I thought that DCHAS-L might know more than we could find on google: We recently purchased a LN2 cryo tank for storing tissue samples. The study requirement is that the tissue samples be stored in the liquid phase of LN2. So far all the folks that I have spoken to around here store in the vapor phase - not liquid. I have been searching online trying to find information on user safety protocols, PPE, and any other helpful pointers for adding and retrieving samples from liquid LN2, but not finding as much details as we would like.

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