From: Russ Phifer <rphifer**At_Symbol_Here**WCENVIRONMENTAL.COM>
Subject: Re: [DCHAS-L] Osmium tetroxide work
Date: January 31, 2012 4:05:44 PM EST
Reply-To: DCHAS-L <DCHAS-L**At_Symbol_Here**MED.CORNELL.EDU>
Message-ID: <B3E67D227FE35F4EBD07E81D2A1064060118BD9839CE**At_Symbol_Here**>

Yung – I have several thoughts on the osmium tetroxide work, particularly in terms of the end product. First, be careful about what buffers your researchers are using. There are about 5 possible buffers for electron microscopy; all are fairly benign except for the one using cacodylic acid. That results in a hazardous waste, whereas the other buffers don’t. And, ….. don’t forget that OSO4 goes for about $15/gram! It can be recycled through a refiner, but not if you degrade it in vegetable oil, and not if you have cacodylic acid buffer.




Russ Phifer

WC Environmental, LLC

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West Chester, PA  19380

610-696-9220x12/ fax 610-344-7519

Cell - 610-322-0657



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From: DCHAS-L Discussion List [mailto:dchas-l**At_Symbol_Here**MED.CORNELL.EDU] On Behalf Of Yung Morgan
Sent: Tuesday, January 31, 2012 3:23 PM
Subject: [DCHAS-L] Osmium tetroxide work


Dear members,


Our researchers heat the 2% solution of Osmium tetroxide in water in a household microwave oven: 2 minutes at 20% or defrost power level. They told me that “everyone” does this.  


Please share with me any thought you have on Osmium tetroxide work? Any special clean up or decon procedures for the chemical hood or in this case the microwave oven?  Anyone had used corn oil for decontamination?


I am writing up a standard operating procedure for this chemical also and would appreciate any suggestion the group may have.  Thank you so much for your help.


Best regards


Yung Morgan, MsPH
Laboratory Safety
Industrial Hygiene Services
Environmental Health and Safety
117 Draper hall
UMASS,Amherst MA 01003
phone (413)  545-2682
Fax  (413) 545-2600
email : pmorgan**At_Symbol_Here**


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