Yung – I have several thoughts on the osmium tetroxide work, particularly in terms of the end product. First, be careful about what buffers your researchers are using. There are about 5 possible buffers for electron microscopy; all are fairly benign except for the one using cacodylic acid. That results in a hazardous waste, whereas the other buffers don’t. And, ….. don’t forget that OSO4 goes for about $15/gram! It can be recycled through a refiner, but not if you degrade it in vegetable oil, and not if you have cacodylic acid buffer.
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Our researchers heat the 2% solution of Osmium tetroxide in water in a household microwave oven: 2 minutes at 20% or defrost power level. They told me that “everyone” does this.
Please share with me any thought you have on Osmium tetroxide work? Any special clean up or decon procedures for the chemical hood or in this case the microwave oven? Anyone had used corn oil for decontamination?
I am writing up a standard operating procedure for this chemical also and would appreciate any suggestion the group may have. Thank you so much for your help.
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