Hello,
As a student finishing my clinical rotation as a medical laboratory scientist, I have received conflicting information regarding the proper disposal of gram stain - Crystal Violet, Gram's Iodine (mordant), Alcohol/Acetone (gen. isopropanol decolourize), and safranin. Some say proper disposal when staining human specimens is sufficient with water flushing down the drain versus collection into a carboy. Since I am still a student in the field, I do not question what is told to me however, I would like to know proper daily disposal. When looking at various MSDS for these chemicals it usually states to follow federal/state/local regulations for empty bottles/excess remainder however, daily disposal seems awfully vague. Can anyone please shed some light on the matter.

Thank you in advance,
Blanca Martinez


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