DCHAS-L Discussion List Archive
From: Beth Shepard <Beth.Shepard**At_Symbol_Here**SIAL.COM>
Subject: Re: [DCHAS-L] working with Ethanedithiol
Date: August 15, 2012 2:51:04 PM EDT
Reply-To: DCHAS-L <DCHAS-L**At_Symbol_Here**MED.CORNELL.EDU>
Message-ID: <61B8FFF7416572438CADB5DF16826AA6061FEDBD**At_Symbol_Here**MADMSG024.promega.com>
That is the recipe my vet gives out for=0A"de-skunking" dogs=2E With the comment for the non-chemists that=0Ait should never be made up ahead of time & put into a closed container=2E=2E=2E=2E
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=0ABeth=0A
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From: =0A Timothy Hauser <Timothy=2EHauser**At_Symbol_Here**PROMEGA=2ECOM>=0A
To: =0A DCHAS-L**At_Symbol_Here**MED=2ECORNELL=2EEDU=0A
Date: =0A 08/15/2012 01:46 PM=0A
Subject: =0A Re: [DCHAS-L]=0Aworking with Ethanedithiol=0A
Sent by: =0A DCHAS-L Discussion=0AList <dchas-l**At_Symbol_Here**MED=2ECORNELL=2EEDU>=0A
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We have used a fresh solution of 1 quart 3% hydrogen=0Aperoxide (drug store strength), =BC cup baking soda and 1 teaspoon of liquid=0Adishwashing soap to neutralize beta-mercaptoethanol contaminated material=2E=0AI also use it on my dogs when they've lost an argument to a skunk and they=0Alose every time=2E
=0A
=0ATimothy M=2E Hauser, CHMM
=0AManager, Environmental, Health & Safety
=0APromega Corporation
=0A2800 Woods Hollow Road, Madison WI 53711 USA
=0ATimothy=2EHauser**At_Symbol_Here**promega=2Ecom | Direct (608) 298-4807 | Cell: (262) 844-1665=0A| Fax: (608) 277-2677
=0A
=0A-----Original Message-----
=0AFrom: DCHAS-L Discussion List [mailto:dchas-l**At_Symbol_Here**MED=2ECORNELL=2EEDU]=0AOn Behalf Of Kristi Ohr
=0ASent: Wednesday, August 15, 2012 1:22 PM
=0ATo: DCHAS-L**At_Symbol_Here**MED=2ECORNELL=2EEDU
=0ASubject: Re: [DCHAS-L] working with Ethanedithiol
=0A
=0AHi Yung,
=0A
=0APaul is right on=2E I've always used a 1:2 (volume:volume) household=0Ableach:water solution for thiols=2E
=0A
=0AKristi
=0A
=0A________________________________________
=0AFrom: DCHAS-L Discussion List [dchas-l**At_Symbol_Here**MED=2ECORNELL=2EEDU] on behalf of Paul=0AHarrison [pharriso**At_Symbol_Here**UNIVMAIL=2ECIS=2EMCMASTER=2ECA]
=0ASent: Wednesday, August 15, 2012 2:04 PM
=0ATo: DCHAS-L**At_Symbol_Here**MED=2ECORNELL=2EEDU
=0ASubject: Re: [DCHAS-L] working with Ethanedithiol
=0A
=0AI am not surprised that no-one will touch the thiol-bearing glassware!
=0A
=0AYou need a hood; don't even think of a glove bag, they are under positive=0Apressure, which will just pump out more stench=2E
=0A
=0AFor clean-up, have a bucket of dilute bleach to hand, IN THE HOOD=2E Put=0Aeverything in it and let it soak=2E Yes, the pipettes, tips, glassware,=0Aand I also have dropped my used gloves in the bleach if in any doubt about=0Atheir state of cleanliness=2E In summary, NOTHING leaves the hood!
=0A
=0AI also advise checking where on the roof your hood vents=2E If the=0Awind is blowing the wrong way, the vented air can be sucked back into the=0Abuilding air inlet=2E My lab has had this happen with tert-butanethiol;=0Athe result is that the building smells the same as it would in the case=0Aof a gas leak, since this thiol is what is used to make natural gas smell=2E=0A Result? Someone pulls the fire alarm, the engines roll in=2E=2E=2E=2E=0A and one's popularity plummets to a new low=2E
=0A
=0AGood luck!
=0A
=0APaul
=0A
=0AOn Wed, 15 Aug 2012 12:17:54 -0400
=0A Yung Morgan <pmorgan**At_Symbol_Here**EHS=2EUMASS=2EEDU> wrote:
=0A> Dear members,
=0A>
=0A> Hope the Summer is finding you well and somewhat rested(?)=2E A question=0Acame from our sister school re: working with Ethane dithiol=0Ain a glove box=2E Any thought on this? Also what kind of decontamination=0Aprocedures for glassware before disposing? We usually find glass=0Aonly boxes full of smelly broken glass and our custodial group would=0Arefuse to remove them=2E
=0A>
=0A> Again thank you so much for any thought you may have=2E Continue=0Adoing great work and enjoying the last of Summer=2E
=0A>
=0A> Best wishes
=0A>
=0A> Yung Morgan, MsPH
=0A> Laboratory Safety
=0A> Industrial Hygiene Services
=0A> Environmental Health and Safety
=0A> 117 Draper hall
=0A> UMASS,Amherst MA 01003
=0A> phone (413) 545-2682
=0A> Fax (413) 545-2600
=0A> email : pmorgan**At_Symbol_Here**ehs=2Eumass=2Eedu
=0A> IH motivation: saving=0Athe world, one fume hood at the time=2E YM
=0A
=0APaul Harrison
=0AAssociate Professor of Chemistry
=0ADepartment of Chemistry and Chemical Biology
=0AMcMaster University
=0A1280 Main St=2E W=2E, Hamilton, ON, L8S 4M1, Canada
=0APhone: (905)525-9140 ext=2E 27290; FAX: (905)522-2509
=0A=0A
=0A=0AThis message and any files transmitted with it are the property of=0ASigma-Aldrich Corporation, are confidential, and are intended=0Asolely for the use of the person or entity to whom this e-mail is=0Aaddressed=2E If you are not one of the named recipient(s) or=0Aotherwise have reason to believe that you have received this=0Amessage in error, please contact the sender and delete this message=0Aimmediately from your computer=2E Any other use, retention,=0Adissemination, forwarding, printing, or copying of this e-mail is=0Astrictly prohibited=2E
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