I recommend BSL2 standard microbiological practices, Safety equipment and Facility standards, paying particular attention to safe work practices with sharps, mucus membrane protection and appropriate disinfection and decontamination. I would also make sure to train lab personnel that any exposed skin of lab personnel should be devoid of cuts, rash etc.
OSHA bloodborne pathogens training is a must along with exposure control manual update on an yearly basis. Plus, I would make sure that the lab personnel complete the biosafety training and the protocol specific training and all training gets documented.
Vijayasmitha Rayadurg DVM, PhD, CBSP
IHRC Contractor for DSR, CDC.
Good Afternoon, Here is a question for the list serve -More and more laboratories are using cancer cells. The body of evidence states that you can not get cancer from touching or even eating a HeLa cell or some other cancerous cell from another species, but I am not sure that I can just swallow that without any reasoning. Anyone have an opinion or reasoning regarding contracting cancer from a cancer cell culture?A cancerous cell has been changed by some virus that is no longer present and the cancer cell is not able to re assemble the virus or affect the genome of another cell.Does anyone have any references for working with cancer cells? Does working with these cells require anything more than BLS-1 or BSL-2? Must these be deactivated before discarding?If anyone has any thoughts or resources regarding working with HeLa or other human and animal cancer cell lines above and beyond what you would do with normal healthy cells, I am listening. Some staff seems to be trivializing this and I am not sure that I agree, I am looking for some reinforcement (on their opinion or mine) and any references or resources in regards to working with cancer cell lines in the lab.Thanks,James Saccardo, MS, CHMMThe College of Staten IslandOffice of Environmental Health and Safety"An ounce of prevention is worth a pound of cure."Benjamin FranklinConfidentiality Note: The information contained in this Email and/or document(s) attached is for the exclusive use of the individual named above and may contain confidential, privileged and non-disclosable information. This communication may also contain data subject to U.S. export laws, not releasable to Foreign Persons unless authorized by 22 CFR 120-130 or 15..CFR 730-774. If so, that data subject to the International Traffic in Arms Regulation cannot be disseminated, distributed or copied to foreign nationals, residing in the U.S. or abroad, absent the express prior approval of the U.S. Department of State. If you are not the intended recipient, you are hereby notified that you are strictly prohibited from reading, photocopying, distributing or otherwise using this Email or its contents in any way. If you have received this communication in error, please notify the sender by reply e-mail and destroy the e-mail message and any physical copies made of the communication.
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