From: Charu Rayadurg <charu3.rayadurg**At_Symbol_Here**GMAIL.COM>
Subject: Re: [DCHAS-L] Working safely with cancer cells
Date: Tue, 17 May 2016 19:03:18 -0400
Reply-To: DCHAS-L <DCHAS-L**At_Symbol_Here**MED.CORNELL.EDU>
Message-ID: CAEGhhsO=WUtrq2MBeruaih-zvR8VgE48Et15x=6WvsevvKSCnw**At_Symbol_Here**
In-Reply-To <579eda819f1a4a66a4795073ca4d0268**At_Symbol_Here**MBOX-01.FLAS.CSI.CUNY.EDU>


I recommend BSL2 standard microbiological practices, Safety equipment and Facility standards, paying particular attention to safe work practices with sharps, mucus membrane protection and appropriate disinfection and decontamination. I would also make sure to train lab personnel that any exposed skin of lab personnel should be devoid of cuts, rash etc.

OSHA bloodborne pathogens training is a must along with exposure control manual update on an yearly basis. Plus, I would make sure that the lab personnel complete the biosafety training and the protocol specific training and all training gets documented.


Vijayasmitha Rayadurg DVM, PhD, CBSP
Biosafety Officer
IHRC Contractor for DSR, CDC.
Atlanta, GA

On May 17, 2016 4:06 PM, "James Saccardo" <James.Saccardo**At_Symbol_Here**> wrote:
Good Afternoon, Here is a question for the list serve -
More and more laboratories are using cancer cells. The body of evidence states that you can not get cancer from touching or even eating a HeLa cell or some other cancerous cell from another species, but I am not sure that I can just swallow that without any reasoning. Anyone have an opinion or reasoning regarding contracting cancer from a cancer cell culture?
A cancerous cell has been changed by some virus that is no longer present and the cancer cell is not able to re assemble the virus or affect the genome of another cell.
Does anyone have any references for working with cancer cells? Does working with these cells require anything more than BLS-1 or BSL-2? Must these be deactivated before discarding?
If anyone has any thoughts or resources regarding working with HeLa or other human and animal cancer cell lines above and beyond what you would do with normal healthy cells, I am listening. Some staff seems to be trivializing this and I am not sure that I agree, I am looking for some reinforcement (on their opinion or mine) and any references or resources in regards to working with cancer cell lines in the lab.
James Saccardo, MS, CHMM
The College of Staten Island
Office of Environmental Health and Safety
"An ounce of prevention is worth a pound of cure."
Benjamin Franklin
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