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Subject: Re: [DCHAS-L] Dilute Ethidium Bromide Disposal in California
Date: Friday, August 10, 2018 at 11:38:25 AM
Author: Penny Manisco <pmanisco**At_Symbol_Here**G.HMC.EDU> 		Next by Date:
Subject: [DCHAS-L] CHAS at a Glance for the Boston ACS Meeting
Date: Fri, 10 Aug 2018 14:07:28 -0400
Author: DCHAS Membership Chair

From: Lawrence M Gibbs <lgibbs**At_Symbol_Here**STANFORD.EDU>
Subject: Fwd: [DCHAS-L] [DCHAS-L] Dilute Ethidium Bromide Disposal in California
Date: Fri, 10 Aug 2018 17:36:34 +0000
Reply-To: ACS Division of Chemical Health and Safety <DCHAS-L**At_Symbol_Here**PRINCETON.EDU>
Message-ID: A94B3393-361C-44C3-926C-0C6830645723**At_Symbol_Here**stanford.edu
In-Reply-To <9DA38AF6-B0D6-4051-A873-A0DBFA279659**At_Symbol_Here**stanford.edu>


Forwarded from Craig Barney. Environmental programs manager at Stanford EHS 

Larry

Begin forwarded message:

From: "Craig A. Barney" <cbarney**At_Symbol_Here**stanford.edu>
Date: August 10, 2018 at 10:23:20 AM PDT
To: Lawrence M Gibbs <lgibbs**At_Symbol_Here**stanford.edu>
Subject: Re: [DCHAS-L] Dilute Ethidium Bromide Disposal in California

Our limit is 0.4% by weight based on the California Toxicity characteristic 
The controversy hinges on not the toxicity but mutagenic property 
Craig

Sent from my toaster

On Aug 10, 2018, at 10:07 AM, Lawrence M Gibbs <lgibbs**At_Symbol_Here**stanford.edu> wrote:

Did'nt stanford do some testing on this many moons ago? 

Larry

Begin forwarded message:

From: Penny Manisco <pmanisco**At_Symbol_Here**G.HMC.EDU>
Date: August 9, 2018 at 4:37:42 PM PDT
To: <DCHAS-L**At_Symbol_Here**PRINCETON.EDU>
Subject: [DCHAS-L] Dilute Ethidium Bromide Disposal in California
Reply-To: ACS Division of Chemical Health and Safety <DCHAS-L**At_Symbol_Here**PRINCETON.EDU>

Hello All,

There seems to be varying opinion on how to dispose of very dilute solutions of ethidium bromide. The concentration in question is 0.5 micrograms/ml of buffer. By hazardous waste definition, this  would not be considered a hazardous waste, but I believe some of our California universities and colleges recommend filtration of the ethidium bromide before disposal at any concentration.

Any guidance would be very much appreciated.

Sincerely,

--
Penny Manisco,
Chemical Hygiene Officer
Harvey Mudd College

(909)6074217
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