Dear Rachel,

       Your waste bottle and all other chemicals not in immediate use belong in a fume hood.  Acetonitrile is flammable and can cause health effects. Other LC mobile phase solvents can be similarly dangerous; always read the MSDS. I hope this is helpful.

Sincerely,

Dr. Bob Butler

STEM and Chem LLC

rgbutler**At_Symbol_Here**verizon.net

We have used acetonitrile routinely with our UPLCs for years. Our waste bottle is in a stable  secondary container in the floor. We have not had issues with fumes. 

Sylvia Tarin Brousseau 

Southwest Laboratory 

Sent from my iPhone

On Jun 2, 2026, at 9:54 AM, Rachel A Kaiser <0000244c46d98b14-dmarc-request**At_Symbol_Here**lists.princeton.edu> wrote:



Hello!

We are hoping to do some work with the LC this summer using acetonitrile but are concerned about ventilation of the fumes. Our waste currently runs to a bottle that has a closed funnel. Do labs typically have their LC waste container in a fume hood for ventilation? Our LC is currently in a fairly small room that has one large vent in the ceiling. Any thoughts or recommendations would be greatly appreciated!

Thank you,

Rachel Kaiser ’22 (she/her/hers)

Chemistry Lab Coordinator | Chemical Hygiene Officer

Wittenberg University

(937)327-6442

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