Date: Wed, 27 Jul 2011 13:38:41 -0400
Reply-To: DCHAS-L Discussion List <DCHAS-L**At_Symbol_Here**LIST.UVM.EDU>
Sender: DCHAS-L Discussion List <DCHAS-L**At_Symbol_Here**LIST.UVM.EDU>
From: Ralph B Stuart <rstuart**At_Symbol_Here**CORNELL.EDU>
Subject: PCR benchtop ventilation?

I was touring some labs yesterday and noticed a variety of small benchtop m
achines that were designed to do Polymerase Chain Reaction chemistry. Some 
of the references I reviewed on this technique listed a variety of hazardou
s chemicals associated with this process (phenol, chloroform, etc.), but th
ey are somewhat dated and I wonder if these machines use these chemicals. 

The immediate question is what level of ventilation these devices need. Fro
m what I can see in the instrument manuals I downloaded, the ventilation ne
eds seem to be limited to temperature control considerations, but I thought
 I would check with the DCHAS list to see if anyone has experience with che
mical exhausts from these systems.

Thanks for any help with this question.

- Ralph

Ralph Stuart CIH
Laboratory Ventilation Specialist

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