From: "Jeskie, Kimberly B." <jeskiekb**At_Symbol_Here**ORNL.GOV>
Subject: Re: [DCHAS-L] Gram Staining information
Date: April 7, 2012 8:31:45 PM EDT
Reply-To: DCHAS-L <DCHAS-L**At_Symbol_Here**MED.CORNELL.EDU>
Message-ID: <A4BDFFCAC336824B8501F8FA6E1DA2D4AD96EC34AD**At_Symbol_Here**>


Waste disposal is really State and even municipality dependent for situations like this and may be why you are receiving conflicting information. We would collect it for disposal through a vendor, but that's us. You really have to ask the person responsible for the disposal process at your institution, and unfortunately you may have to ask again when you move on to another institution.


Sent with Good (

-----Original Message-----
From: Martinez, Blanca A. [BMARTINEZ4**At_Symbol_Here**MY.APSU.EDU]
Sent: Saturday, April 07, 2012 02:33 PM Eastern Standard Time
Subject: [DCHAS-L] Gram Staining information

As a student finishing my clinical rotation as a medical laboratory scientist, I have received conflicting information regarding the proper disposal of gram stain - Crystal Violet, Gram's Iodine (mordant), Alcohol/Acetone (gen. isopropanol decolourize), and safranin. Some say proper disposal when staining human specimens is sufficient with water flushing down the drain versus collection into a carboy. Since I am still a student in the field, I do not question what is told to me however, I would like to know proper daily disposal. When looking at various MSDS for these chemicals it usually states to follow federal/state/local regulations for empty bottles/excess remainder however, daily disposal seems awfully vague. Can anyone please shed some light on the matter.

Thank you in advance,
Blanca Martinez

Sent from my iPhone

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