From: "Ferm, Barret" <fermbarreta**At_Symbol_Here**SAU.EDU>
Subject: Re: [DCHAS-L] Gram Staining information
Date: April 7, 2012 9:33:18 PM EDT
Reply-To: DCHAS-L <DCHAS-L**At_Symbol_Here**MED.CORNELL.EDU>
Message-ID: <9BD19E74-2E3D-42EC-ADE3-7C8049E5A37B**At_Symbol_Here**MY.APSU.EDU>

Thank you for asking your question, Blanca. I, too, am interested in hearing replies to it. For these types of solutions I have been advised to add bleach to decolorize, and adjust pH to (5 - 8) if necessary, then down the drain. Is this okay??

Barry Ferm

On Sat, Apr 7, 2012 at 1:00 PM, Martinez, Blanca A. <BMARTINEZ4**At_Symbol_Here**> wrote:
As a student finishing my clinical rotation as a medical laboratory scientist, I have received conflicting information regarding the proper disposal of gram stain - Crystal Violet, Gram's Iodine (mordant), Alcohol/Acetone (gen. isopropanol decolourize), and safranin. Some say proper disposal when staining human specimens is sufficient with water flushing down the drain versus collection into a carboy. Since I am still a student in the field, I do not question what is told to me however, I would like to know proper daily disposal. When looking at various MSDS for these chemicals it usually states to follow federal/state/local regulations for empty bottles/excess remainder however, daily disposal seems awfully vague. Can anyone please shed some light on the matter.

Thank you in advance,
Blanca Martinez

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