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Previous by Date: Subject: Re: [DCHAS-L] Update on theatrical blood bath Date: December 18, 2012 8:08:16 AM EST Author: Karen Salazar |
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Monona et al:
The CDC will give you more insight than I could provide; however, regarding sterilization by heat: microbiological growth media must be sterilized by pressurized
steam sterilization (autoclaving) at 121=B0 C at 15 psi (sorry for mixing English with metric units: the autoclave temp/pressure is almost a litany in microbiology!) The reason is that some bacteria (some of which are pathogenic) produce endospores, a dormant
state that can withstand extremes of environmental conditions (including boiling temperatures). If the media has already supported microbial growth, pressurized sterilization must occur for an hour (generally).
Sounds like you might want to put on
Bye Bye Birdie or some other less gory production instead!
Edward J. McGrath
Science Supervisor
Red Clay Consolidated School District
1502 Spruce Avenue
Wilmington, DE 19805
(302) 552-3768
"Fortune favors the prepared mind." Louis Pasteur
From: DCHAS-L Discussion List [mailto:dchas-l**At_Symbol_Here**MED.CORNELL.EDU]
On Behalf Of Karen Salazar
Sent: Tuesday, December 18, 2012 8:08 AM
To: DCHAS-L**At_Symbol_Here**MED.CORNELL.EDU
Subject: Re: [DCHAS-L] Update on theatrical blood bath
Monona,
I hope you are able to find a solution now that the CDC is on the case; however, if you plan to use heat as the solution, I just wanted to remind you to take precautions concerning possible mechanical failure of the pumping system as well.
Good Luck,
Karen
On Dec 18, 2012, at 5:08 AM,
ACTSNYC**At_Symbol_Here**CS.COM wrote:
All,
I can't thank you all enough. As you may remember we were discussing 1500 gallons of theatrical blood that was going to be pumped from six 275 gallon tanks into a wading pool on stage about every three days, exposed to air and feet, and back into the tanks
again repeatedly for about 6 weeks.
After reading through all of the answers, I decided there probably was no disinfectant system that would work due to the 10% loading of organic chemicals in the water and heat needed to be explored as an option. The question now is the temperature. Sterilization
of water requires a rolling boil, but ain't no way those temps can be achieved under the conditions at the theater's holding tanks. What about pasteurization temperatures? Would they be enough? I've now even heard about strange thingies called hemophyllic
actinomycetes that survive all kinds of heat. But I'm not a bug person so I called on the CDC for help.
This is to let you all know I got the call yesterday and the CDC is on the case. I talked at length to the person leading this charge and it was confirmed we were right: there is no disinfectant system that will hold under these conditions without also being
toxic to the waders and even to the breathers of air in the theater.
I will let you know what happens. Again: many thanks.
Monona
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