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Gas Regulators


  1. Introduction
  2. Select the Proper Regulator
  3. Installing and Using a Regulator
  4. Disconnecting a Regulator
  5. Further Information


An entire web site could be devoted to explaining the various kinds of regulators (high pressure, corrosive gas, fluorine etc.) that are available! This page is not meant to be comprehensive, merely an introduction to regulators you might find in a typical inorganic or organic laboratory. If you are ever unsure about how to use a regulator or another piece of equipment, ask your laboratory supervisor or safety expert.

There are typically a wide variety of gas regulators in your average chemistry department. The majority of these are single stage and two stage regulators such as the one shown below.

a gas regulator

The advantage of a two stage regulator is that the pressure flow remains consistent until the tank is nearly empty. Therefore, you might want to use a double stage regulator on a gas chromatograph (GC), but for a typical Schlenk line a single stage regulator would suffice.

Two other common types of "regulators" that you might encounter are actually called flow control valves. Unlike a regulator, these DO NOT control pressure, only flow. However, they permit one to easily dispense gas from a cylinder. As they lack gauges, be extremely cautious when hooking a flow valve up to a vacuum line!!

The manual flow control valve shown below on the left is usually used on small cylinders (carbon dioxide, ethylene etc.) and the one on the right is typically used on lecture bottles. Small single and double stage lecture bottle regulators are also available.

a manual flow valve        a lecture bottle valve

Finally, note that you should NEVER use grease or oil on a regulator. Not only will it gunk up the inside and contaminate your reaction system, but these organic materials can react with the gas being dispensed. Never use an oxygen regulator for other gases. Cross-contamination of internal parts (especially with grease or oil) could cause a rapid oxidation and fire.

Select the Proper Regulator

Not all regulators can be used on all cylinders. For example, flammable gases such as hydrogen require brass fittings. The Compressed Gas Association (CGA) has devised a system that ensures accidental mix-ups can not occur. Each cylinder and regulator have connection fittings that are designated by a CGA number. Below are some common CGA numbers. High pressure tanks or lecture bottles require different fittings:

GasesCGA Connection #
Carbon dioxide320
Boron trifluoride, hydrogen chloride, hydrogen bromide, hydrogen iodide, hydrogen sulfide, silicon tetrafluoride 330
Carbon monoxide, ethylene, hydrogen, hydrogen selenide350
Acetylene, allene, butadiene, butane, butenes, cyclopropane, dimethylether, methane, propane, propylene, vinyl methyl ether510
Argon, nitrogen, helium, noble gases580
Air (industrial grade)590
Boron trichloride, chlorine, nitric oxide, nitrogen dioxide, nitrogen trioxide, sulfur dioxide, phosphorous pentafluoride, many halocarbons660
Anyhdrous ammonia705

CGA numbers are typically (but not always) stamped on the regulator just above the threads of the cylinder connection. Some will even state specifically which gas(es) for which they can be used. It is a very bad idea alter a regulator or use an adapter to "make" a regulator fit a tank for which it was not designed. This kind of deliberate tampering with a safety feature could lead to a serious accident.

Deadly!Here's an example where someone hooked a nitrogen tank to an oxygen supply line, resulting in the deaths of 3 people. This is not an isolated example, either.Deadly!

Flammable gases have reverse threads meaning that the connection is tightened by turning the nut counterclockwise. You can always tell a reverse thread connection because the nut that you tighten has a line inscribed around its circumference. Compare the nut on the manual flow control valve (reverse threaded) with the one on the double stage regulator (regular thread) shown above.

Installing and Using a Regulator

  1. Make sure your cylinder is properly secured, that you have the correct regulator and that you are aware of any special hazards of the gas you are working with.

  2. Remove the cylinder valve cap (counterclockwise). Place it somewhere nearby.

  3. Some regulators (on lecture bottles and certain corrosive gases) require a Teflon or lead washer to be inserted between the tank outlet and regulator. Check to see if this is required before continuing.

  4. Make sure that the regulator outlet valve (A) is shut. Screw it clockwise until it seats. Do not overtighten it or you can damage the valve seat.

    a gas regulator

  5. Make sure that the regulator control valve (B) is shut. Screw it counterclockwise until it is almost completely unscrewed. If you unscrew it completely, just put it back in.

  6. Screw the regulator onto the tank by hand until it is almost finger tight. Some people like to use Teflon tape on this connection, but that's generally not a good idea. Bits of Teflon tape can get blown into the regulator, causing a leak, valve malfunction or erroneous reading.

  7. What you do next depends on the kind of gas you are working with and whether you need to exclude air from the gas line you're connecting to. Aldrich Chemical discusses this in more detail in their Technical Bulletin AL-151, Gas Equipment Configurations.

    For nitrogen and argon cylinders:

    For corrosive or reactive gases:

    For flammable gases:

  8. Open the tank valve slowly (counterclockwise). Watch the tank pressure on the regulator (C).

  9. Slowly turn the regulator control valve (B) until the regulator pressure (D) is at the desired level.

  10. Open the regulator outlet valve (A). You can regulate flow with this valve, but the ultimate pressure depends on the setting of the regulator control valve!

  11. Check your system for leaks using Snoop (a commercial product) or some soapy water. Snoop is preferred since it leaves no residue. If you find leaks and tightening the connections does not help ask your instructor for assistance.

    Reminder: Do not use Teflon tape on Swagelock ferrule compression fittings.

Disconnecting a Regulator

  1. Shut the tank valve on the gas cylinder.

  2. Slowly open the outlet valve (A) on the regulator .

  3. Watch the pressure gauges C and D drop to zero.

  4. Open the regulator control valve (B) (turn it clockwise) to ensure that all pressure has been released.

  5. If you were using a corrosive gas, purge the system with a dry inert gas.

  6. Using a wrench (not pliers!) disconnect the regulator from the gas cylinder. Replace the protective cylinder cap immediately.

  7. If your regulator was used with a corrosive gas, purge it again with dry air or nitrogen in the hood for several minutes.

  8. If your cylinder is empty, it must be properly labeled and then returned to the manufacturer or distributor (in many cases, this is your school or company stockroom). Do not store empty gas cylinders in the laboratory.

    Reminder: Make sure the tank valve is closed whenever you are not dispensing gas through the regulator.

Further Information

cover illustration
Handbook of
Compressed Gases

by Compressed Gas Association
& Debbie Angerman


This page was last updated Monday, March 30, 2015.
This document and associated figures are copyright 1996-2015 by Rob Toreki. Send comments, kudos and suggestions to us via email.